實驗地點:肇慶市疾病預防控制中心
肇慶市疾病預防控制中心是原肇慶市衛生防疫站以及肇慶市職業病防治院、端州、鼎湖、大旺高新區衛生防疫站的基礎上組建而成,屬社會公益性衛生事業單位。2002年12月26日掛牌成立。承擔著全市390萬人口的疾病預防控制、免疫規劃、職業病防治、衛生監測、衛生檢驗、健康教育、科研培訓、突發公共衛生事件應急處理等工作職能,直管端州、鼎湖、大旺三區疾病控制和衛生監測、檢驗工作,是全市疾病預防控制和衛生檢測業務技術指導中心。
實驗:老鼠耳朵提RNA
老鼠耳朵研磨提取RNA,用于做后續基因、鼠疫病毒檢測;因為老鼠耳朵含筋、毛發、皮膚,所以相對比較難磨,用戶之前用手工配合液氮研磨,如此一來樣本損失比較多,而且研磨過程中存在污染導致樣本活性降解。上海凈信全自動樣品快速研磨儀可批量處理樣本,每個樣本都是獨立離心管,不會交叉污染,用戶可批量化抽提基因組,大大提高了工作效率。
實驗設備:
品牌:上海凈信;名稱:全自動樣品快速研磨儀;型號:JXFSTPRP-24L
實驗步驟:
準備:待研磨老鼠耳朵樣本,離心管以及不銹鋼研磨珠若干顆,清洗并滅菌;金屬適配器放入-80度冰箱預冷凍10分鐘。
1、待研磨樣本裝入離心管,加入清洗并滅菌后的不銹鋼研磨珠,加入試劑
2、將裝有樣品離心管裝入上海凈信全自動樣品快速研磨儀,注意水平配平
3、鎖緊鎖扣,設置參數;
4、啟動研磨,待研磨完成后取出樣品如下圖;

結語:用戶傳統的方式是手工研磨,因為樣本體積小,手工研磨樣本損失比較嚴重,提出的RNA濃度非常低,所以用戶最終選擇上海凈信全自動樣品快速研磨儀,樣本零損失,而且操作簡單便捷,可以批量處理,用戶對上海凈信全自動樣品快速研磨儀評價非常高。
儀器介紹:

上海凈信?的JXFSTPRP-L?系列組織研磨儀(專利產品,仿冒必究!ZL2019301918692)是一種特殊的、快速的、高效率的、多試管的一致系統。它能將任何來源(包括土壤、植物和動物的組織/器官、細菌、酵母、真菌、孢子、古生物標本等)的原始DNA、RNA和蛋白質進行提取和純化。 將樣品和研磨球放入研磨儀內(配置研磨罐或離心管/適配器),在高頻擺動作用下,研磨球在研磨儀內來回高速撞擊及摩擦,在非常短的時間內便可完成樣品的研磨、粉碎、混合及細胞破壁。
性能特點:
1.穩定性好:采用三維一體8字形振蕩方式,研磨更充分,穩定性更好;
2.效率高:1分鐘內完成2×24、2×48、4×96個樣品的研磨;
3.重復性好:同一組織樣本設定相同程序,獲得相同的研磨效果;
4.操作簡便:內置程序控制器,可對研磨時間、轉子的振動頻率等參數進行設置;
5.安全性高:具備安全罩及安全鎖;
6.無交叉污染:在研磨過程中處于全封閉狀態,避免交叉污染;
7.低噪音:儀器運行過程中,噪音小于55dB,不會對其它實驗或儀器產生干擾。
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Non-contact shaped DNA interrupter
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Rotary mixer HS-5
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Split ultrasonic cell disruptor
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Nitrogen Blowing Apparatus
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QuEChERS sample preparation system
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Three-dimensional high-speed vibration ball mill
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Three-head shock type high-energy ball mill
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Fast tissue low-temperature crushing homogenizer
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Multifunctional homogenizer
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Multi-sample tissue grinder-96L
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3D Centrifugal Cryogenic Grinder
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Vertical oscillator
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Constant temperature and humidity weighing system
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Water quality chlorophyll grinder
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Mortar grinder
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High-efficiency vibrating disc grinder
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Fully automatic cryogenic sample processing system
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Automatic sample rapid grinder
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Multi-sample tissue grinder-64L
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Multi-channel microbial magnetic bead homogenizer
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Automatic sample rapid grinding machine-64
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Automatic sample rapid grinder-96
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Automatic sample rapid grinder-192
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Three-dimensional mixer
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Molecular hybridization furnace
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Cryogenic Grinder JXFSTPRP-CLN
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Cryogenic tissue grinder
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Cryogenic tissue grinder
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High-speed cryogenic grinding homogenizer
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Semi-automatic hammer cyclone mill
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High-speed tissue grinding machine KZ-II
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Automatic sample rapid grinding machine
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Automatic sample rapid grinder-24
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QuEChERS pre-processing all-in-one machine
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Immersion liquid nitrogen Cryogenic Grinder
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Automatic liquid nitrogen freezing grinding machine
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Cryogenic Grinder (Semi-automatic Liquid Nitrogen Cryogenic Grinder)
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3D sample tissue grinder
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Cryogenic Grinding Machine
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Automatic hammer cyclone mill
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Multi-sample Tissue Grinder-24L
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Constant temperature mixer
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Beating sample homogenizer
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Beating aseptic homogenizer
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Jaw Crusher
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Automatic homogenizer
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Single cell suspension preparation instrument
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Single cell preparation instrument (temperature control)
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Biological indicator incubator
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Dry thermostat
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Knife grinder
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Constant temperature mixer (refrigeration)
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Microplate Heat Sealer
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Microplate Oscillator
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Oscillator special for microplate
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Microplate incubator
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Microplate incubator
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Thermostatic metal bath
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Temperature-controlled single cell suspension preparation instrument
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High-speed grinding homogenizer-192
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Bone and Teeth Grinder
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Frozen Bone Teeth Crusher
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Basic cryogenic grinder
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Cryogenic grinder
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Cryogenic tissue grinder
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3D Centrifugal Cryogenic Grinder
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Cross-beating grinder
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Gene amplification instrument PCR instrument THT-96G (gradient type)
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Automatic rotating sampler
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Flip oscillator
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Fully Automatic Flip Oscillator
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Fully automatic low temperature flip oscillator
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Handheld Grinder
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High Power Handheld Grinder
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Aquatic product animal husbandry solid phase extraction device
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Gradient amplification PCR detection analyzer THG-96G (gradient)
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3D Cryogenic Grinder
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High temperature four-module thermostatic metal bath
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Microplate Shaker
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High energy ball mill
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Low-Temperature Cyclone Hammer Mill
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MINI rapid detection grinder
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High Throughput Tissue Grinder-2018
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High temperature type CNC super constant temperature bath
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Solid Phase Extraction Apparatus
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Solid phase extraction device
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Grinding bead homogenizer
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Beating aseptic homogenizer
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Fully automatic homogenizer
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New type soil drying box
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High Throughput Tissue Grinder - GTL64
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In situ hybridization
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High Throughput Grinder
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Medium Flux Grinder
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High-throughput sample rapid grinder
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Ultra High Throughput Tissue Grinder
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Ultra High Throughput Tissue Grinder
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UV crosslinker
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Three-dimensional mixer
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Sample pre-processing machine
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