大腸桿菌在自然環境中,飼料、飲水、動物的體表、孵化場、孵化器等各處普遍存在,是由大腸桿菌埃氏菌的某些致病性血清型菌株引起的疾病總稱,也由一定血清型的致病性大腸桿菌及其毒素引起的一種腸道傳染病。此次實驗室里的老師需要用快速樣品研磨儀來研磨菌類提取蛋白,比如大腸桿菌、芽孢桿菌等類。
實驗地點:中南民族大學
實驗材料和儀器:
樣品:大腸桿菌
儀器:上海凈信全自動樣品研磨儀(JXFSTPRP-24L)

耗材:離心管,氧化鋯珠
實驗步驟:
1.準備2ml*24適配器,鋼珠
2.將裝好的樣品規范放入JXFSTPRP-24研磨儀中
批量研磨樣品,一般情況下,由于客戶研磨的是菌類,組織偏小,研磨管內配比較為講究,設置參數
3.得到如圖所示研磨樣品
實驗過程、結果圖示:

如上圖為我們的實驗材料:

測試中
注意:
1、每支離心管樣品量一般不超過離心管體積的三分之二。
2、樣品盡量對稱放置,以保證研磨時的受力平衡。
3、珠子和液體比例1:1或者1:2效果最好,珠子比例1:1放入
儀器介紹:
上海凈信®的JXFSTPRP-L®系列組織研磨儀(專利產品,仿冒必究!ZL2019301918692)是一種特殊的、快速的、高效率的、多試管的一致系統。它能將任何來源(包括土壤、植物和動物的組織/器官、細菌、酵母、真菌、孢子、古生物標本等)的原始DNA、RNA和蛋白質進行提取和純化。將樣品和研磨球放入研磨儀內(配置研磨罐或離心管/適配器),在高頻擺動作用下,研磨球在研磨儀內來回高速撞擊及摩擦,在非常短的時間內便可完成樣品的研磨、粉碎、混合及細胞破壁。
性能特點:
1.穩定性好:采用三維一體8字形振蕩方式,研磨更充分,穩定性更好;
2.效率高:1分鐘內完成2×24、2×48、4×96個樣品的研磨;
3.重復性好:同一組織樣本設定相同程序,獲得相同的研磨效果;
4.操作簡便:內置程序控制器,可對研磨時間、轉子的振動頻率等參數進行設置;
5.安全性高:具備安全罩及安全鎖;
6.無交叉污染:在研磨過程中處于全封閉狀態,避免交叉污染;
7.低噪音:儀器運行過程中,噪音小于55dB,不會對其它實驗或儀器產生干擾。
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